課程名稱 |
膜蛋白動力學 Membrane Protein Kinetics |
開課學期 |
107-1 |
授課對象 |
生物資源暨農學院 獸醫學研究所 |
授課教師 |
蔡沛學 |
課號 |
VM7098 |
課程識別碼 |
629EM2650 |
班次 |
|
學分 |
2.0 |
全/半年 |
半年 |
必/選修 |
選修 |
上課時間 |
|
上課地點 |
|
備註 |
本課程以英語授課。英文授課。 限碩士班以上 總人數上限:8人 外系人數限制:2人 |
Ceiba 課程網頁 |
http://ceiba.ntu.edu.tw/1071VM7098_ |
課程簡介影片 |
|
核心能力關聯 |
本課程尚未建立核心能力關連 |
課程大綱
|
為確保您我的權利,請尊重智慧財產權及不得非法影印
|
課程概述 |
1. Course Introduction
2. The Structure and the Properties of Biological Membrane
3. Membrane proteins (I)
4. Membrane proteins (II)
5. Moving Proteins into Membranes
6. Endocytosis of Membrane Proteins
7. Mechanisms for Protein Translocation (I)
8. Mechanisms for Protein Translocation (II)
9. Mid-term week (suspend)
10. Guest lecture
11. Isolation and Visualization of Membrane Proteins (I)
12. Isolation and Visualization of Membrane Proteins (II)
13. Recent Breakthrough Technologies for Studying Membrane Protein (including literature discussion)
14. Experimental Designs for Membrane Proteins
15. Lab Practice I (Isolation of Membrane Proteins)
16. Lab Practice II (Visualization of Membrane Proteins)
17. Problems Shooting/Discussion (whole class)
18. Final Report |
課程目標 |
We aim to allow our students to have the ability to properly design, correctly precede specific experiments when they deal with membrane proteins and related subjects. In class, we combine in class lecture, lab practice (allow students to work on their own specific experiments that directly linked to their master or PhD study) as well as interactive discussions in order to have direct and fast feedback on questions and difficulties they encounter. |
課程要求 |
active participate class lectures, discuss difficulties you encounter for your project with lecturer |
預期每週課後學習時數 |
|
Office Hours |
|
指定閱讀 |
待補 |
參考書目 |
1.Molecular Biology of the Cell (B. Albert et. al, 5th edition), Garland Science
2.Molecular Cell Biology (H. Lodish et al, 7th edition), Freeman
3.The Molecular Probes Handbook (I. Johnson, M.T.Z Spence et. al 11th edition, Invitrogen) |
評量方式 (僅供參考) |
|
週次 |
日期 |
單元主題 |
第2週 |
10/5 |
Introduction |
第3週 |
10/12 |
Experimental Consult I |
第4週 |
10/19 |
Visualisation and cell membrane acquisition |
第5週 |
10/26 |
Biomembrane structure |
第6週 |
11/2 |
Experimental Consult II |
第7週 |
11/9 |
Moving proteins into membrane |
第8週 |
11/16 |
Experimental Consult III |
第9週 |
11/23 |
Vesicular Traffic, Secretion, and Endocytosis |
第10週 |
11/30 |
Experimental Consult IV |
第11週 |
12/7 |
Protein Translocation I |
第12週 |
12/14 |
Protein Translocation II |
第13週 |
12/21 |
Experimental Consult V |
第14週 |
12/28 |
Experimental Consult VI |
|